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  • Title: [Induction of transposon Tn1 translocation in UV-irradiated Escherichia coli cells].
    Author: Smirnov SP, Tarasov VA.
    Journal: Genetika; 1981; 17(3):420-3. PubMed ID: 6265313.
    Abstract:
    Ampicillin transposon Tn1 translocates from plasmid RP4 into E. coli chromosome with a frequency of about 3.10(-4) per cell. Irradiation of bacteria with UV-light increases the frequency of translocation essentially. Mutation in lexA gene controlling the expression of cell UV-inducible functions blocks the induction of transposon translocation. Protein synthesis inhibitor--chloramphenicol and transcription inhibitor--rifampicin decrease the effect of UV-light on transposition process. A possible mechanism of inducible transposition is discussed.
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