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  • Title: Synthesis of a model promoter for gene expression in Escherichia coli.
    Author: Dobrynin VN, Korobko VG, Severtsova IV, Bystrov NS, Chuvpilo SA, Kolosov MN.
    Journal: Nucleic Acids Symp Ser; 1980; (7):365-76. PubMed ID: 6265875.
    Abstract:
    We have synthesized a 67-membered double-stranded DNA containing putative initiation sites for transcription and translation in E. coli. Among the potential applications of this DNA are construction of artificial genes, cloning of foreign genes in E. coli, and study of structure - activity relations in promoter and ribosome binding sites.
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