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  • Title: A new solvent system for the separation of neutral glycosphingolipids.
    Author: Watanabe K, Arao Y.
    Journal: J Lipid Res; 1981 Aug; 22(6):1020-4. PubMed ID: 6268725.
    Abstract:
    A solvent system and a column for high performance liquid chromatography for the separation of glycosphingolipids without derivatization is described. A column pakced with porous silica gel (latrobeads) and eluted with a mixture of isopropanol-hexane-water with increasing water content and decreasing hexane content was used. Glycosphingolipids with mono- to dodeca- or tetrakaidecasaccharides were separated within 60 min and the separation pattern was highly reproducible. The method was applied for preparative separation of highly complex glycolipids with blood group activity.
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