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  • Title: Enzymatic properties of the purified putA protein from Salmonella typhimurium.
    Author: Menzel R, Roth J.
    Journal: J Biol Chem; 1981 Sep 25; 256(18):9762-6. PubMed ID: 6270101.
    Abstract:
    In the previous paper (Menzel, R., and Roth, J. (1981) J. Biol. Chem. 256, 9755-9761) we have described the purification of a protein, the putA gene product, which has both proline oxidase and pyrroline-5-carboxylic acid dehydrogenase activities. In this paper we demonstrate that these enzyme activities are distinct with respect to a number of characteristics. The oxidase activity proceeds by a ping-pong mechanism involving the reduction of an enzyme-bound flavin. The dehydrogenase activity utilizes an ordered reaction mechanism.
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