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  • Title: Simultaneous isolation of bovine eosinophils and neutrophils on gradients of Percoll.
    Author: Riding GA, Willadsen P.
    Journal: J Immunol Methods; 1981; 46(1):113-9. PubMed ID: 6270212.
    Abstract:
    Bovine eosinophils and neutrophils have been isolated from venous blood using a two-step procedure which involves initial preparation of a granulocyte-rich fraction by centrifugation and osmotic shock followed by sensity gradient centrifugation on Percoll. Good yields of both cell types are obtained with purities of 90% or better. The isolated cells are viable and actively phagocytic. The effects of phagocytosis on the metabolism of carbon-1 of d-glucose and the incorporation of iodine have been measured and several differences between bovine cells and those of other species were noted.
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