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  • Title: A simple method for the isolation of basolateral plasma membrane vesicles from rat kidney cortex. Enzyme activities and some properties of glucose transport.
    Author: Inui K, Okano T, Takano M, Kitazawa S, Hori R.
    Journal: Biochim Biophys Acta; 1981 Sep 21; 647(1):150-4. PubMed ID: 6271206.
    Abstract:
    A procedure for preparing basolateral membrane vesicles from rat renal cortex was developed by differential centrifugation and Percoll density gradient centrifugation, and the uptake of D-[3H] glucose into these vesicles was studied by a rapid filtration technique. (Na+ + K+)-ATPase, the marker enzyme for basolateral membranes, was enriched 22-fold compared with that found in the homogenate. The rate of D-glucose uptake was almost unaffected by Na+ gradient (no overshoot).
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