These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.
Pubmed for Handhelds
PUBMED FOR HANDHELDS
Search MEDLINE/PubMed
Title: Properties of calcium ionophore-induced generation of superoxide anion by human neutrophils. Author: Smith RJ, Iden SS. Journal: Inflammation; 1981 Sep; 5(3):177-92. PubMed ID: 6271675. Abstract: Exposure of human neutrophils to the calcium ionophore, A23187, eventuates in a time- and concentration-dependent generation of superoxide anion (O2-) in the presence but not absence of extracellular calcium. The selective requirement for calcium is demonstrated by the observation that magnesium caused a dose-related inhibition of A23187-stimulated O2- generation. Preincubation of neutrophils with cytochalasin B prior to their interaction with A23187 results in a significant enhancement of O2- production. The activity of the O2--generating system was maximum at 37 degrees C and was significantly curtailed at lower and higher temperatures. A23187-induced O2- generation was inhibited by the sulfhydryl reagents. N-ethyl maleimide (NEM) and iodoacetic acid (IA), and by the metabolic inhibitor 2-deoxy-D-glucose (2-DG) in the absence of glucose and cytochalasin B. Cyanide was inactive. Therefore, A23187 represents a useful pharmacologic probe for investigating the divalent cation and metabolic requirements of the neutrophil O2--generating system.[Abstract] [Full Text] [Related] [New Search]