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  • Title: Contrasting patterns of protein kinase activities among normal peripheral blood lymphocyte subpopulations and lymphoid cell lines.
    Author: Elias L, Longmire J, Bankhurst AD.
    Journal: J Lab Clin Med; 1982 Jan; 99(1):56-63. PubMed ID: 6274984.
    Abstract:
    Human peripheral mononuclear cell suspensions were separated into E-rosette-positive (T cell) and E-rosette-negative (non-T cell) fractions and assayed for cAMP binding activity and histone and casein kinase activity, in the presence and absence of cAMP. Each of these activities was higher in E-rosette-positive than E-rosette-negative fractions. Cyclic AMP binding and histone kinase activities were further noted to be higher in T lymphoblastoid cell lines when compared to lines of non-T lymphoid cell origin. In addition. DEAE-cellulose chromatography revealed a qualitative contrast of isoenzymic composition of protein kinase from these cell lines. Cyclic AMP-independent casein kinase activity was high in both types of cell lines. These results suggest that high protein kinase activity is characteristic of T cells and may be related to their higher sensitivity to cAMP agonists in comparison to non-T cells. This study also provides further evidence for an association between increased cAMP-independent protein kinase activity and the transformed state, which has been noted for a variety of other cell types.
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