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  • Title: [Purine catabolism in rat brain (author's transl)].
    Author: Franco R, Canela EI, Bozal J.
    Journal: Rev Esp Fisiol; 1981 Sep; 37(3):255-62. PubMed ID: 6275465.
    Abstract:
    In soluble rat brain fraction, the specific activities of purine nucleoside phosphorylase, guanine deaminase, 5'Nucleotidase and adenosine deaminase, decrease in their mentioned order. A kinetic parameter comparison between these enzymes shows that 5'Nucleotidase with AMP has the lowest KM and the greatest Vmax values, while purine nucleoside phosphorylase has its lowest KM and its greatest Vmax values with guanosine and with inosine, respectively. The enzymes activity is not modified by the metabolic intermediates differently from their own reaction products which behave as competitive inhibitors.
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