These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Localization of synaptic and nonsynaptic nicotinic-acetylcholine receptors in the goldfish retina.
    Author: Zucker C, Yazulla S.
    Journal: J Comp Neurol; 1982 Jan 10; 204(2):188-95. PubMed ID: 6276449.
    Abstract:
    The localization of nicotinic-cholinergic receptors in the inner plexiform layer (IPL) of goldfish retina was studied by electron microscopic analysis of the binding pattern of a conjugate or horseradish peroxidase and alpha bungarotoxin (HRP-alpha BTx). Specific HRP reaction product (blockade by 1mM curare) was found at both synaptic and nonsynaptic sites. Synaptic binding sites for HRP-alpha BTx, which accounted for only 16% of the total specific reaction product sites, always involved an amacrine process as the presynaptic element, whereas amacrine, ganglion, and bipolar cells could be post-synaptic elements at labeled synapses. Only 17.5% of the total number of amacrine synapses were labeled by HRP-alpha BTx. Labeled synapses showed the same distribution in the IPL as unlabeled synapses: bimodal for amacrine-to-bipolar synapses with peak concentrations at the 20% and 80% layers and unimodal for amacrine-to-nonbipolar synapses with a peak concentration at the 60% layer. Nonsynaptic binding sites for HRP-alpha BTx (84% of total) were seen on the dendrites of ganglion, amacrine, and bipolar cells. The distribution of the nonsynaptic sites in the IPL largely accounts for the trilaminar binding pattern of 125I-alpha BTx as observed in light microscopic autoradiographs. If, as appears likely, the distribution of synapses is the relevant variable in determining the sites of neuronal interaction for a given transmitter system, then this study further illustrates the importance of distinguishing synaptic from nonsynaptic binding when using receptor-ligand probes to localize sites of chemical synaptic transmission.
    [Abstract] [Full Text] [Related] [New Search]