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  • Title: Immunization of mouse spleen cell cultures in the absence of serum and its proteins using SiO2 and 2-mercaptoethanol.
    Author: Burger M.
    Journal: Immunology; 1982 Feb; 45(2):381-5. PubMed ID: 6277774.
    Abstract:
    High recoveries of plaque-forming cells were obtained in a serum-free medium using 2-mercaptoethanol and SiO2. Under such conditions an increase in the number of plaque-forming cells occurred between the fourth and fifth day of incubation, as compared with cultures with foetal calf serum. The addition of glutathione to the medium had a stimulating effect, it was however not absolutely necessary for a positive response. The recovery of plaque-forming cells was strongly influenced by an interdependence of cell density and amount of SiO2 gel particles.
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