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  • Title: Molecular cloning of the tolC locus of Escherichia coli K-12 with the use of transposon Tn10.
    Author: Morona R, Reeves P.
    Journal: Mol Gen Genet; 1981; 184(3):430-3. PubMed ID: 6278256.
    Abstract:
    We have cloned the tolC gene of E. coli K-12 into pSF2124 by using transposon Tn10 as the marker to first isolate the relevant DNA fragment. The gene is on a 10.5 kb EcoRI fragment, and Tn5 insertion mutagenesis locates the gene near one end of this EcoRI fragment. An EcoRI-PstI fragment has been subcloned into pBR322 to facilitate further analysis of the gene.
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