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  • Title: Primary structure of Escherichia coli RNA polymerase nucleotide substitution in the beta subunit gene of the rifampicin resistant rpoB255 mutant.
    Author: Ovchinnikov YuA, Monastyrskaya GS, Gubanov VV, Lipkin VM, Sverdlov ED, Kiver IF, Bass IA, Mindlin SZ, Danilevskaya ON, Khesin RB.
    Journal: Mol Gen Genet; 1981; 184(3):536-8. PubMed ID: 6278262.
    Abstract:
    The transducing phage lambda dsupM814 and the plasmid pIB1830 containing the wild-type rpoB gene have been constructed and the primary structure of the gene's central fragment has been established. In contrast with the wild-type, the gene of the rpoB255 mutant, whose primary structure has been published, was found to contain an A.T. leads to T.A. transversion entailing the substitution of a valine residue for the aspartic acid residue (516) of the wild-type beta subunit.
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