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  • Title: Production of a T-antigen-related protein in mammalian cells after stable transformation with a cloned SV40 gene fragment.
    Author: Horst J, Jacob E, Weckler C, Deppert W.
    Journal: Hoppe Seylers Z Physiol Chem; 1982 Apr; 363(4):445-8. PubMed ID: 6281154.
    Abstract:
    A recombinant plasmid based on pBR322 has been constructed which carries the replicator proximal early region of SV40 DNA, including the viral origin of replication (ORI). It lacks a major part of the tumour antigen 3'-coding region, the large T-antigen termination codon and the polyadenylation site. The recombinant plasmid was transferred together with the herpes simplex virus thymidine kinase gene, as a selectable marker into mouse LTK- cells. Integration and expression of the cloned SV40 gene fragment in TK+ transformants could be demonstrated by DNA restriction and blot hybridization and by immunofluorescence techniques.
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