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  • Title: Adaptation of human neutrophil responsiveness to the chemoattractant N-formylmethionylleucylphenylalanine. Heterogeneity and/or negative cooperative interaction of receptors.
    Author: Seligmann BE, Fletcher MP, Gallin JI.
    Journal: J Biol Chem; 1982 Jun 10; 257(11):6280-6. PubMed ID: 6281264.
    Abstract:
    The dose-response characteristics of the neutrophil 3-3'-dipentyloxacarbocyanine (di-O-C5(3)) fluorescence response to repetitive stimulation with the chemoattractant N-formylmethionylleucylphenylalanine (fMet-Leu-Phe) were studied. Neutrophils exposed to the chemoattractant fMet-Leu-Phe at less than 5 X 10(-8) M subsequently responded only to higher concentrations of fMet-Leu-Phe. This stimulus-induced modification of neutrophil responsiveness involved a reversible fMet-Leu-Phe-induced shift in response Km (the concentration of fMet-Leu-Phe producing a half-maximal response) to higher values which occurred 1 to 2 min after exposure to fMet-Leu-Phe and represented a form of adaptation. A Hill coefficient of 0.68 +/- 0.07 was determined from analysis of the data indicating that the di-O-C5(3) fluorescence response behavior is compatible with functional negatively cooperative interaction and/or heterogeneity of fMet-Leu-Phe receptors. In related studies, analysis of the binding of fMet-Leu-[3H]Phe to intact cells and cell-free plasma membrane preparations resulted in Hill coefficients of 0.64 +/- 0.06 and 0.69 +/- 0.07, respectively, indicating that fMet-Leu-Phe binding exhibits properties similar to the fMet-Leu-Phe-elicited di-O-C5(3) fluorescence response. Modulation of receptor affinity, through either negative cooperativity or changing populations of heterogeneous receptors, may be an important mechanism by which neutrophils adapt and respond to a gradient of chemoattractant during the process of chemotaxis.
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