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  • Title: [Kinetic characteristics of microsomal NAD-glycohydrolase natural and solubilized with a non-ionic surface-active substance].
    Author: Sestini S, Cinci G, Ricci C.
    Journal: Boll Soc Ital Biol Sper; 1982 Apr 30; 58(8):450-2. PubMed ID: 6284185.
    Abstract:
    Microsomal rat spleen NAD-glycohydrolase was solubilized by Nonidet P40. The solubilized enzyme shows Nicotinamide inhibition and pH dependence at the same extent as unsolubilized microsomal one. It differs from the latter in having a higher affinity for NAD and NADP, and in showing two peaks, instead of one, on electrofocusing: the former with a pH 5 pI without any activity, the latter with a pH 4, 1 pI with a high NAD-ase activity.
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