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  • Title: [Cytochromes c and P-450 as terminal acceptors in a reconstituted system of mitochondrial hydroxylation].
    Author: Armenian AG, Mardanian SS, Nalbandian RM.
    Journal: Biokhimiia; 1982 May; 47(5):784-90. PubMed ID: 6284260.
    Abstract:
    Both cytochromes c and P-450 can be reduced in vitro by a mitochondrial hydroxylation chain consisting of flavoprotein, adrenodoxin and NADPH as electron donors. The effects of pH, ionic strength, content and stoichiometry of intermediate electron carriers on the reduction rate of cytochromes c and P-450 have been compared. The data obtained demonstrate that the reduction of these cytochromes proceeds by different ways. It has been found that cytochrome c has an inhibitory effect on cytochrome P-450 reduction. On the other hand, cytochrome P-450 remarkably activates the process of cytochrome c reduction.
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