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  • Title: [Cloning of the promoter of the RNA polymerase operon located before the structural gene of the beta-subunit].
    Author: Bass IA, Kurenova EV, Mekhedov SL.
    Journal: Mol Biol (Mosk); 1982; 16(3):575-80. PubMed ID: 6285178.
    Abstract:
    An EcoRI-fragment of the rpoBC operon carrying the end of the rplL gene, the intercistron region and the beginning of the rpoB gene cloned on the pBRH4 plasmid makes it tetracyclin-resistant, i. e. possesses the properties of a promoter. There is no appreciable difference in the degree of antibiotic resistance of recombinant plasmids carrying the main PJ promoter and the additional promoter.
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