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Title: A zymodeme study of Entamoeba histolytica in a group of South African schoolchildren. Author: Sargeaunt PG, Williams JE, Jackson TF, Simjee AE. Journal: Trans R Soc Trop Med Hyg; 1982; 76(3):401-2. PubMed ID: 6287686. Abstract: Using a biphasic culture medium, stocks of intestinal amoebae were isolated from a group of children attending school in Durban, South Africa. These were compared with stocks collected in other areas of the world already characterized using the electrophoretic patterns of four enzymes: glucose phosphate isomerase (GPI), phosphoglucomutase (PGM) L-malate: NADP+ oxido-reductase (oxalacetate-decarboxylating) (ME) and hexokinase (HK). 33% of 94 samples grew Entamoeba histolytica, only one of which gave a pattern indicative of a pathogenic stock. Entamoeba hartmanni, Dientamoeba fragilis and Entamoeba coli were also grown from some samples, increasing the total positive samples for all species isolated to 40%.[Abstract] [Full Text] [Related] [New Search]