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  • Title: [Isolation of a membranous fraction of toad gastric mucosa].
    Author: García de Bohórquez E, Martínez M, Chacín J.
    Journal: Acta Physiol Lat Am; 1980; 30(3):153-8. PubMed ID: 6287808.
    Abstract:
    In the present work the isolation and some characteristics of a membrane fraction from toad gastric mucosa are described. By combining differential centrifugation in a discontinuous gradient of sucrose it was possible to obtain a band at the interphase of 22% sucrose 37%, whose enzymatic and electron microscopic analysis indicated to be a fraction enriched of luminal plasma membranes from oxyntic cells, with very poor contamination by other subcellular organelles. This is a suitable membrane preparation that can be used to perform studied on the mechanisms of acid secretion in toads.
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