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  • Title: [Effect of cyclic adenosine-3',5'-monophosphate, chloramphenicol and actinomycin D on Gluconobacter oxydans biosynthesis of extracellular levansaccharase].
    Author: Elisashvili VI.
    Journal: Mikrobiologiia; 1982; 51(3):436-42. PubMed ID: 6289057.
    Abstract:
    The biosynthesis of levansucrase by Gluconobacter oxydans was shown to be induced in media containing sorbitol or fructose. An addition of glucose at a concentration of 0.1% to the culture growing in a medium with sorbitol stimulated the biosynthesis of levansucrase, whereas glucose at a concentration of 0.5-0.6% inhibited the enzyme synthesis by 20-30%. Gluconic acid, a product of glucose metabolism, also repressed the synthesis of levansucrase, but to a lesser degree than glucose. Cyclic adenosine-3',5-'monophosphate eliminated the repression by glucose added to a medium with sorbitol. An addition of chloramphenicol to the culture growing in a medium with sorbitol showed that the induction of levansucrase required de novo protein synthesis. The inhibiting action of chloramphenicol increased with its concentration. An increase in the concentration of actinomycin D from 5 to 200 micrograms/ml inhibited the bacterial growth by 50%, but stimulated the biosynthesis of levansucrase by 40%.
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