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  • Title: Structure at restriction endonuclease MboI cleavage sites protected by actinomycin D or distamycin A.
    Author: Nilsson MG, Skarped C, Magnusson G.
    Journal: FEBS Lett; 1982 Aug 23; 145(2):360-4. PubMed ID: 6290273.
    Abstract:
    Restriction endonuclease MboI cleavage of DNA was inhibited by actinomycin D and distamycin A. The two inhibitors protected different subsets of the 8 cleavage sites in polyoma DNA. The cleavage reactions were analyzed both in the presence of minimal inhibitory concentrations of the compounds and at higher concentrations, allowing cleavage at only 1 site/DNA molecule. The experiments showed that cleavage sites most efficiently protected by actinomycin D had putative inhibitor binding sites at a distance of 1-2 base pairs from the MboI recognition sequence. Distamycin A, in contrast, apparently has to bind immediately adjacent to the MboI recognition sequence to protect from cleavage.
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