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  • Title: Analysis of buoyant density of canine peripheral blood leukocytes with PVP-Silica (Percoll) density gradients.
    Author: Buurman WA, Vegt PA, Groenewegen G, van der Linden CJ, Jeunhomme GM.
    Journal: Vet Immunol Immunopathol; 1982 Nov; 3(6):547-56. PubMed ID: 6294965.
    Abstract:
    Isolation of canine peripheral blood mononuclear cells with a one step centrifugal separation procedure has not been very successful sofar. Significant contamination with polymorphonuclear cells has been reported. An analysis of the buoyant density of canine peripheral blood leukocytes on a self-generating Percoll gradient showed that the buoyant densities of polymorphonuclear cells and lymphocytes are so near that separation with high purity and yield is not possible with the use of a density gradient. Transient changes in buoyant density of polymorphonuclear cells have been observed. In such situations differences in buoyant density between cell types have been observed which permit separation of mononuclear cells from polymorphonuclear cells at a reasonable yield.
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