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  • Title: Location of the single gene for elongation factor Tu on the Euglena gracilis chloroplast chromosome.
    Author: Passavant CW, Stiegler GL, Hallick RB.
    Journal: J Biol Chem; 1983 Jan 25; 258(2):693-5. PubMed ID: 6296078.
    Abstract:
    The structural gene for elongation factor Tu (EF-Tu) has been mapped by heterologous hybridization to a 2900 base pair sequence of Euglena gracilis Klebs Strain Z Pringsheim chloroplast DNA within the EcoRI fragment, Eco N. The hybridization probes were obtained from a HhaI restriction fragment containing internal sequences to Escherichia coli EF-Tu, located in the tufA gene locus, and from an EcoRI restriction fragment of chloroplast DNA from the eukaryotic algae Chlamydomonas reinhardii, containing the 3' end of the chloroplast EF-Tu gene. This is the second identified protein gene locus to be mapped on the E. gracilis chloroplast genome, and the first using prokaryotic DNA as a probe.
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