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Title: Binding of carcinoembryonic antigen (CEA) to an anti-CEA immunosorbent in the presence of detergents. Author: Wagener C, Schwonzen M, Breuer H. Journal: J Clin Chem Clin Biochem; 1982 Nov; 20(11):787-9. PubMed ID: 6296267. Abstract: The binding of [125I]CEA to an anti-CEA Sepharose-4B immunosorbent has been investigated in the presence of detergents. Non-ionic detergents (Triton-X-100, Lubrol PX and Tørgitol NPX) at concentrations of 5-40 milligrams did not inhibit the binding of [125I]CEA to the immunosorbent. Sodium deoxycholate decreased the binding from 98% (concentration of detergent 0.6 milligram) to 41% (concentration of detergent 20 milligrams). Sodium dodecyl sulfate inhibited the binding of [125I]CEA to the immunosorbent considerably, even at low concentrations.[Abstract] [Full Text] [Related] [New Search]