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  • Title: Dependence on pH of substrate binding to lactose carrier in Escherichia coli cytoplasmic membranes.
    Author: Yamato I, Rosenbusch JP.
    Journal: FEBS Lett; 1983 Jan 10; 151(1):102-4. PubMed ID: 6297984.
    Abstract:
    Lactose permease in Escherichia mediates proton-substrate cotransport. The molecular mechanism of this process is not understood. We examined the effect of proton concentration on the binding of a substance analogue to the carrier. The dissociation constant of p-nitrophenyl-alpha-galactoside from the carrier was dependent on pH, with an apparent pKa of 9.7.
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