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  • Title: Gene mapping of rotavirus double-stranded RNA segments by northern blot hybridization: application to segments 7, 8, and 9.
    Author: Dyall-Smith ML, Azad AA, Holmes IH.
    Journal: J Virol; 1983 Apr; 46(1):317-20. PubMed ID: 6298465.
    Abstract:
    Cloned DNA copies of double-stranded RNA segments 7, 8, and 9 of UK bovine rotavirus were nick-translated with [alpha-(32)P]ATP and hybridized to double-stranded RNA of various rotavirus strains which had been separated on long polyacrylamide gels and then transferred to o-aminophenylthioether paper. Specific hybridization of the UK calf clones to the separated RNA segments allowed the corresponding genes of four different rotaviruses to be rapidly determined.
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