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  • Title: Site-directed point mutation in the src gene oF rous sarcoma virus results in an inactive src gene product.
    Author: Bryant D, Parsons JT.
    Journal: J Virol; 1983 Mar; 45(3):1211-6. PubMed ID: 6300458.
    Abstract:
    Site-directed mutagenesis techniques were used to construct defined point mutations within the src gene of the Prague A strain of Rous sarcoma virus. Bisulfite mutagenesis at a Bg/I restriction site in the src gene yielded three mutations which contained the same single base change, a guanine-to-adenine transition. The resulting genomes encoded an src protein containing a substitution of threonine for alanine at amino acid position 433. Transfection of chicken cells with mutagenized DNA did not result in cellular transformation even though the cells produced a pp60src. Immune complexes containing mutant pp60src did not phosphorylate immunoglobulin G heavy chain or casein.
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