These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Verapamil enhances the efficiency of DNA-mediated gene transfer in mammalian cells.
    Author: Akiyama S, Ono M, Kuwano M.
    Journal: Biochem Biophys Res Commun; 1983 Feb 10; 110(3):783-8. PubMed ID: 6301463.
    Abstract:
    Treatment of Ltk- cells with the calcium antagonists, verapamil and diltiazem, but not nifedipine, causes a 3-fold enhancement of the frequency of transfer of the cloned gene for herpes simplex virus thymidine kinase (HSV-tk). The frequency of phenotypic expression of the HSV-tk DNA was 20 to 34 times higher than that of genotypic transformation. Phenotypic expression was also 2.3 to 2.6 times increased when 20 micrograms/ml of verapamil was present during calcium phosphate-mediated DNA transfection.
    [Abstract] [Full Text] [Related] [New Search]