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  • Title: A vector that uses phage signals for efficient synthesis of proteins in Escherichia coli.
    Author: Queen C.
    Journal: J Mol Appl Genet; 1983; 2(1):1-10. PubMed ID: 6302192.
    Abstract:
    I have developed a plasmid vector pCQV2 for high-level expression of proteins in E. coli. The plasmid contains a very strong promoter and translation start point from bacteriophage lambda, and a restriction endonuclease site in which a gene may be placed under control of these initiation signals. The plasmid also contains the gene for a temperature-sensitive repressor of the lambda promoter, allowing 100-fold induction of protein expression. The vector pCQV2 has been used to synthesize SV40 small t antigen at the level of 5-10% of total E. coli protein, representing an order-of-magnitude improvement over previous methods.
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