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  • Title: Detection of Clostridium perfringens enterotoxin by tissue culture and double-gel diffusion methods.
    Author: Giugliano LG, Stringer MF, Drasar BS.
    Journal: J Med Microbiol; 1983 May; 16(2):233-7. PubMed ID: 6302258.
    Abstract:
    Culture filtrates of four enterotoxin-producing strains of Clostridium perfringens type A produced morphological changes in cells of 10 of 12 established mammalian cell lines tested, and particularly Vero (African green-monkey kidney) and MDCK (dog kidney) cells. Double-gel diffusion and Vero-cell tests detected enterotoxin in culture filtrates of, respectively, 29 (28%) and 56 (54%) of 103 strains of C. perfringens tested. The feasibility of cell-culture assays for the routine detection of C. perfringens enterotoxin is indicated.
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