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  • Title: Immunocytochemical visualization of luteinizing hormone-human chorionic gonadotropin (LH-hCG) receptors in Leydig cells in primary culture.
    Author: Begeot M, Mombrial C, Dubois PM, Dubois MP, Dazord A, Haour F.
    Journal: J Histochem Cytochem; 1983 Jul; 31(7):898-904. PubMed ID: 6304183.
    Abstract:
    Visualization of human chorionic gonadotropin (hCG) binding sites was obtained by immunocytochemical reaction on Leydig cells cultured in chemically defined medium. After a short in vitro incubation with hCG or luteinizing hormone (LH), the cells were fixed and the bound molecules were revealed using anti-hCG or anti-LH antisera. In both cases the immunocytochemical reaction appeared as granulations at the cell surface. After prolonged (48 hr) culture in the presence of 0.5, 5, or 50 ng/ml of hCG, the hormone receptor complex is still visible. Similarly, following short exposure to hCG (0.5 or 50 ng/ml) and one or two days of culture without hCG, the immunocytochemical reaction is still present. These observations suggest that the half-life of the bound hormone is very long. This in vitro system in which the amount and time of hormone exposure are precisely defined provides arguments in favor of a long-term maintenance of the receptor complexes at the cell surface.
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