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Title: Characterization by molecular cloning of insertion mutants in TOL catabolic functions. Author: Lehrbach PR, Jeenes DJ, Broda P. Journal: Plasmid; 1983 Mar; 9(2):112-25. PubMed ID: 6304792. Abstract: A physical and genetic map of the Tol catabolic region of pWWO (TOL) was obtained by restriction endonuclease analysis of several DNA insertion mutants (xylA, xylA xylS, xylS, and xylR) of R plasmid--TOL derivatives. In two cases, the inserted DNA was shown from restriction, DNA hybridization, or heteroduplex analysis of cloned Hind III fragments to originate from within pWWO fragment Hind III-E. The effect of these DNA insertions on Tol catabolic activity and on structural alterations to the TOL plasmid is discussed.[Abstract] [Full Text] [Related] [New Search]