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  • Title: Isolation of horse mononuclear cells, especially of monocytes, on Isopaque-Ficoll neutral density gradient.
    Author: Bruyninckx WJ, Blancquaert AM.
    Journal: Vet Immunol Immunopathol; 1983 May; 4(4):493-504. PubMed ID: 6306903.
    Abstract:
    Horse mononuclear cells were separated from whole blood using neutral density gradient centrifugation on Isopaque-Ficoll. The resulting cell suspension was comparable in composition with similarly prepared human and bovine mononuclear cell preparations. The relative concentration of monocytes was increased by the use of a gradient with density lower than that originally proposed by Böyum (Böyum, A. 1968. Scand. J. Clin. Lab. Investig. 21 supple. 97:77-89). Contamination by neutrophils was limited either by using a gradient medium of lower density or by replacing Isopaque-Ficoll by Percoll-0.9% NaCl. Although the density of the Isopaque-Ficoll appears to be the main determinant in the isolation method of Böyum, the mechanism of separation of the cell population is complex and a substantial variability of the results can be expected.
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