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  • Title: Cloning and physical mapping of the cysB region of Salmonella typhimurium.
    Author: Jagura-Burdzy G, Kredich NM.
    Journal: J Bacteriol; 1983 Aug; 155(2):578-85. PubMed ID: 6307971.
    Abstract:
    The cysB region of Salmonella typhimurium was cloned in pBR322 and localized to a 1.75-kilobase HincII fragment. Two-dimensional protein electropherograms showed levels of the cysB polypeptide chain that were several fold higher in plasmid-bearing strains than in the wild type. Fully derepressed levels of sulfite reductase and O-acetylserine sulfhydrylase in cysB plasmid-bearing strains were only 25% higher than in the wild type, suggesting that the product of this regulatory gene ordinarily is not a limiting factor in the expression of the cysteine regulon. The mapping of cysB deletions by Southern blots showed a good correlation between the genetic and the physical maps of this gene. The supX gene was initially cloned with cysB and is within 0.7 kilobase of cysB.
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