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  • Title: Differences in the physiological response and ultrastructure of human and guinea pig vas deferens. Effects of prostaglandins, irradiation, and temperature.
    Author: Michailov MC, Murray AB, Zettler F, Grindler-Greimel HW.
    Journal: Urol Int; 1983; 38(4):234-42. PubMed ID: 6308870.
    Abstract:
    Marked differences were observed in the mechanical reactions of human and guinea pig vas deferens to prostaglandins, irradiation, and cooling. In human preparations prostaglandin E1 (0.1-1 ng/ml) had an augmentory effect on the contractile response after electrical neurostimulation (10 Hz, 0.3 ms, 3 s), but no visible influence (at concentrations ranging from 1 ng to 10 micrograms/ml) on the contractile response after electrical muscle stimulation (10 Hz, 40 ms, 3 s). In contrast, in guinea pig preparations (PGE1 (0.1-1 ng/ml) had an inhibitory effect on the contractile response after electrical neurostimulation and an augmentory effect (0.1-1 micrograms/ml) on the contractile response after electrical muscular stimulation. Human vas deferens showed higher radiosensitivity than guinea pig preparations. The neurotransmitters acetylcholine and catecholamines increased the radiosensitivity of guinea pig preparations, but not of human ones. Vas deferens reacted to short-time (15-120 s) cooling with an immediate temporary contraction, at 25 degrees C of short (seconds), at 5 degrees C of long (minutes) duration; after rewarming (5-37 degrees C) a second contraction appeared in guinea pig preparations, but not in human ones. Whereas the contraction to electrical neurostimulation (10 Hz, 0.3 ms, 3 s) was abolished in human preparations by cooling, it was only inhibited in guinea pig vas deferens. Electron microscopy showed differences in the ultrastructure of human and guinea pig vas deferens. Muscle cells were more widely separated in human vas deferens (generally 400 nm or more) than in guinea pig (approximately 100-200 nm), and the intracellular space in human preparations contained more collagen. The axons in human preparations contained predominantly large granular and agranular vesicles, those in guinea pig preparations small granular and agranular vesicles. The possible correlation between the physiological response of human and guinea pig vas deferens and the ultrastructural differences is discussed. The results indicate the possibility that other pharmacophysiological and toxicological phenomena could be essentially different in human and guinea pig material.
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