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  • Title: [Construction of a genome library from a beta-0-thalassemic individual from Ferrara: characterization of clones containing beta globin genes].
    Author: Marchetti G, Evangelisti A, Bernardi F, Perrotta C, Conconi F, Del Senno L.
    Journal: Boll Soc Ital Biol Sper; 1983 Jun 30; 59(6):758-64. PubMed ID: 6309196.
    Abstract:
    A library of genomic DNA was prepared from a patient with beta o Ferrara thalassaemia: random human DNA fragments (15 - 20 Kb) have been joined to phage lambda vectors and cloned has viable phage particles (4). 4x10(5) phages have been screened for their content in beta globin gene sequences, using a human beta cDNA plasmid (5) as hybridization probe. Five positive clones have been isolated and characterized by restriction endonuclease cleavage analysis and by the hybridization experiments. The results obtained allow the precise localization of the human fragments inside the beta like globin gene cluster (6). The comparison of the thalassaemic fragments with the normal DNA (6 - 7) shows two different restriction endonuclease sites, for Xba I and Eco RI respectively, downstream from the human beta globin gene.
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