These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Selective release of the disaccharide 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-D-galactose from epiglycanin by endo-N-acetyl-alpha-D-galactosaminidase.
    Author: Bhavanandan VP, Codington JF.
    Journal: Carbohydr Res; 1983 Jul 16; 118():81-9. PubMed ID: 6311416.
    Abstract:
    Epiglycanin, the major glycoprotein of TA3-Ha mammary carcinoma ascites cells, was radiolabeled with tritium in the terminal D-galactose and 2-acetamido-2-deoxy-D-galactose residues. Alkaline-borohydride treatment, reported to release five O-glycosyl-linked chain types from epiglycanin, resulted in the cleavage of 98-99% of the radioactivity from the protein. Of this, 63% of the radioactivity from epiglycanin and 70% from asialoepiglycanin co-migrated with an authentic sample of 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-D-galactitol on a column of Bio-Gel P-6. Incubation of [3H]galactose-epiglycanin with endo-N-acetyl -alpha-D-galactosaminidase (Diplococcus pneumoniae), and fractionation of the mixture on a column of Bio-Gel P-4, gave only one oligosaccharide peak containing 62 and 70%, respectively, of the radioactivity of epiglycanin and asialoepiglycanin. This oligosaccharide comigrated with authentic 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-D-galactose (1) on columns of Bio-Gel P-2 and P-4 and on paper chromatograms. Results of experiments in which unlabeled epiglycanin was treated with enzyme and the products analyzed, by three different methods, suggested that 78-85% of 1 had been cleaved. Another enzyme, N-acetyl-alpha-D-galactosaminyl-oligosaccharidase from Clostridium perfringens, exhibited similar specificity and cleaved 65% of the radioactivity from ([3H]galactose)asialoepiglycanin, which was eluted from a Bio-Gel P-2 column as the disaccharide 1.
    [Abstract] [Full Text] [Related] [New Search]