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  • Title: Enzyme-linked immunosorbent assay for the detection of Epstein-Barr virus-induced antigens and antibodies.
    Author: Sternås L, Luka J, Kallin B, Rosén A, Henle W, Henle G, Klein G.
    Journal: J Immunol Methods; 1983 Oct 14; 63(2):171-85. PubMed ID: 6311908.
    Abstract:
    Two Epstein-Barr virus (EBV)-specific ELISA tests were developed. One, based on the use of crude extracts from virus producer cells highly induced in the presence of Ara C (providing EA + VCA- cells) or in the absence of the drug (providing EA + VCA + cells) is suitable for the detection of antibodies directed against antigen complexes associated with the lytic virus cycle; i.e., EA, VCA and presumably also MA. The second, performed with purified EBNA, can be used for the detection of antibodies to the transformation-associated nuclear antigen. The tests are expected to find application in the dissection of antibody responses of patients to various antigenic subcomponents, the monitoring of EBV-coded antigens during biochemical purification, and the screening of spent media from hybridoma cultures for EBV-specific antibodies.
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