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  • Title: Reaction of beef plasma and lentil seedlings Cu-amine oxidases with phenylhydrazine.
    Author: Rinaldi A, Floris G, Sabatini S, Finazzi-Agrò A, Giartosio A, Rotilio G, Mondovì B.
    Journal: Biochem Biophys Res Commun; 1983 Sep 30; 115(3):841-8. PubMed ID: 6312997.
    Abstract:
    The reaction of copper amine oxidases from beef plasma and lentil seedlings with hydrazine derivatives has been studied. A 1:1 stoichiometry was always found for the irreversible binding to the dimeric proteins. The formation of the adduct does not require the presence of oxygen or copper. Substrates compete with hydrazine derivatives for the binding to the enzymes. The binding of hydrazines and of substrate has different effects on the EPR spectra of enzymic copper.
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