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  • Title: Identification of the native form of chicken gizzard myosin light chain kinase with the aid of monoclonal antibodies.
    Author: Adachi K, Carruthers CA, Walsh MP.
    Journal: Biochem Biophys Res Commun; 1983 Sep 30; 115(3):855-63. PubMed ID: 6312998.
    Abstract:
    Examination, by immunoblotting, of myosin light chain kinase-containing fractions obtained during purification of the enzyme from chicken gizzard has shown that a single species (Mr = 136,000) exists in the muscle and that this enzyme is degraded, primarily to a 130,000-dalton fragment, during purification. These conclusions were confirmed by phosphorylation of the different species of myosin light chain kinase by the isolated catalytic subunit of cyclic AMP-dependent protein kinase.
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