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  • Title: Quantitative isolation of DNA restriction fragments from low-melting agarose by Elutip-d affinity chromatography.
    Author: Schmitt JJ, Cohen BN.
    Journal: Anal Biochem; 1983 Sep; 133(2):462-4. PubMed ID: 6314845.
    Abstract:
    The use of a disposable affinity column and low-melting-temperature agarose for the quantitative preparation of DNA restriction fragments is presented. After electrophoretic separation of DNA, the band(s) are excised and the DNA/agarose melted in a low-salt buffer. After cooling, the DNA is bound to an Elutip-d affinity column. Fragments are eluted at high salt and concentrated by ethanol precipitation. Recoveries greater than 80% are achieved with purity suitable for most applications in molecular biology.
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