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  • Title: Solubilization of ligand-stabilized vasopressin receptors from plasma membranes of bovine kidney and rat liver.
    Author: Boer R, Crause P, Fahrenholz F.
    Journal: Biochem Biophys Res Commun; 1983 Oct 14; 116(1):91-8. PubMed ID: 6315012.
    Abstract:
    The solubilization of vasopressin receptors from plasma membranes of bovine kidney and rat liver by different detergents was investigated. A prerequisite for the extraction of vasopressin receptors retaining binding affinity for their ligand was the stabilization of the receptors by the prior formation of the membrane-bound hormone-receptor complexes. The vasopressin-receptor complexes from both kidney and liver membranes were solubilized in a high yield with dodecyl-beta-D-maltoside and 3-laurylamido-N,N'-dimethylpropylaminoxide. Several other nonionic detergents including octyl-beta-D-glucopyranoside effectively extracted the hepatic vasopressin receptor. For the hormone-receptor complex solubilized from bovine kidney with dodecyl-beta-D-maltoside, a Stokes' radius of 5.8 nm was determined.
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