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  • Title: Light-activated proton-motive force generation in lipid vesicles containing cytochrome b-c1 complex and bacterial reaction centres.
    Author: Rich PR, Heathcote P.
    Journal: Biochim Biophys Acta; 1983 Nov 30; 725(2):332-40. PubMed ID: 6315060.
    Abstract:
    (1) Purified bovine heart mitochondrial cytochrome b-c1 complex (ubiquinone-cytochrome c oxidoreductase) and photosynthetic reaction centres isolated from Rhodopseudomonas sphaeroides strain R-26 have been incorporated into lipid vesicles. In the presence of cytochrome c and ubiquinone-2, light activation caused a cyclic electron transfer involving both components. (2) Since cytochrome c is added outside the vesicles, it is both reduced by the cytochrome b-c1 complex and oxidised by the reaction centre on the outside of the vesicles. Ubiquinone-2, however, is reduced by the reaction centres at a site in contact with the inside of the vesicles, but the reduced form, ubiquinol-2, is oxidised by the cytochrome b-c1 complex at a site in contact with the outer aqueous phase. (3) In the presence of valinomycin plus K+, initiation of cyclic electron flow causes protons to move from inside the vesicles to the outer medium and the H +/2e- ratio was calculated to be close to 4.
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