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  • Title: [Immobilization of proteins and cell fragments using a new method of microencapsulation].
    Author: Pommerening K, Ristau O, Rein H, Dautzenberg H, Loth F.
    Journal: Biomed Biochim Acta; 1983; 42(7-8):813-23. PubMed ID: 6316945.
    Abstract:
    Cytochrome c, hemoglobin, urease and liver microsomes were microencapsulated in aqueous solution by use of a new method. The membrane of the microcapsules consist of a symplex, which is formed predominantly by electrostatic interactions between a polymeric polyanion and a polymeric polycation. The membrane is characterized by high mechanical stability and is a barrier for globular substances with molecular weights larger than 12000, but permeable for substances with lower molecular weight. The microcapsules contain the protein or microsomes and additionally the polymeric polyanion in the liquid interior. Structure and function of microencapsulated proteins and microsomes are preserved. The developed method of microencapsulation opens new possibilities for the application of biomacromolecules, particularly for extracorporal detoxification.
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