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  • Title: Biochemical and autoradiographic studies of TRH receptors in sections of rabbit spinal cord.
    Author: Sharif NA, Pilotte NS, Burt DR.
    Journal: Biochem Biophys Res Commun; 1983 Oct 31; 116(2):669-74. PubMed ID: 6316961.
    Abstract:
    Receptors for thyrotropin-releasing hormone (pGlu-His-Pro-NH2, TRH) on thaw-mounted sections of rabbit spinal cord have been identified biochemically and visualized by light microscopic autoradiography. Binding of [3H] [3-Me-His2]TRH to 20 microns sections exhibited high apparent affinity and a pharmacological specificity almost identical to that previously demonstrated for spinal TRH receptors in membranes. In autoradiograms, the highest density of TRH receptors appeared in the substantia gelatinosa of the dorsal gray and around the central canal, with intermediate levels in the ventral gray.
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