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  • Title: Improvement of respiratory syncytial virus replication in actively growing HEp-2 cells.
    Author: Pons MW, Lambert AL, Lambert DM, Rochovansky OM.
    Journal: J Virol Methods; 1983 Oct; 7(4):217-21. PubMed ID: 6317708.
    Abstract:
    The growth of respiratory syncytial (RS) and parainfluenza type III (PI3) viruses has been studied in actively growing versus relatively stationary HEp-2 cells. There was no effect on PI3 virus growth. RS virus synthesis was stimulated from 20- to 60-fold by growth in actively growing cultures when the cell density was approximately 1/3 that of a confluent culture. This stimulation was manifested by a greater yield of virus per cell, more virus-specific mRNA produced per cell, and a 50% decrease in the time before cytopathic changes appeared.
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