These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Chromatography and partial purification of solubilized opiate receptors.
    Author: Pfeiffer A.
    Journal: Life Sci; 1983; 33 Suppl 1():203-6. PubMed ID: 6319861.
    Abstract:
    Sheep brain membrane opiate receptors were solubilized using two different approaches: (A) Chaps was used to solubilize [3H]buprenorphine labeled membranes. Macromolecular labeled material had a stokes radius of approximately 90 A. Treatment with phospholipase A2, but not phospholipase C, decreased the stokes radius to 50 A indicating the presence and eventual structural role of phospholipids in the solubilized opiate receptor complex. (B) Digitonin-NaCl was used for solubilization of receptors which were then labeled with [3H]diprenorphine. This complex could be chromatographed on hydroxylapatite, DEAE-sepharose and phenylboronate gels successively permitting an approximately 100-fold purification over the solubilized starting material.
    [Abstract] [Full Text] [Related] [New Search]