These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Cloning and sequencing of the xylose isomerase and xylulose kinase genes of Escherichia coli.
    Author: Lawlis VB, Dennis MS, Chen EY, Smith DH, Henner DJ.
    Journal: Appl Environ Microbiol; 1984 Jan; 47(1):15-21. PubMed ID: 6320721.
    Abstract:
    A 4.2-kilobase-pair fragment of the Escherichia coli chromosome which contains the genes for xylose isomerase and xylulose kinase was cloned into plasmid pBR322. The hybrid plasmid (designated pECX14) complements strains deficient in either or both of the two enzymes. Deletion derivatives of pECX14 were used to localize the two genes on the cloned fragment. The entire nucleotide sequence of the cloned fragment was determined. Open reading frames which, if translated, would encode proteins of molecular weights 54,000 and 52,000 were found. These were identified as the isomerase and kinase structural genes, respectively.
    [Abstract] [Full Text] [Related] [New Search]