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  • Title: Competitive inhibition by the D-isomer in racemic mixtures used as substrate in kinetic studies: a simple method for data treatment.
    Author: Mares-Guia M, Silva E, Tunes H.
    Journal: Arch Biochem Biophys; 1984 Jan; 228(1):278-81. PubMed ID: 6320735.
    Abstract:
    A set of equations was applied that allows the use of racemic mixtures for the estimation of kinetic parameters in systems where the L-isomer is substrate and the D-isomer a competitive inhibitor, displaying data as double reciprocal plots. A statistical treatment was introduced that renders compatible the output of presently available programs with the specific model requirements, with few additional calculations. An example is shown with beta-trypsin and its inhibition of benzozyl-D-arginine p-nitroanilide (Ki = 1.12 mM, pH 8,0, 37 degrees C).
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